Separation of Fluorescence and Light Scattering

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The Zhang Group discovered that both light scattering and fluorescence (not light scattering alone assumed in existing literature) can contribute to the resonance synchronous spectroscopy obtained with fluorescent samples. Presented is a ratiometric Bandwidth Varied Polarized Resonance Synchronous Spectroscopy (BVPRS2) method for separating fluorescence and scattering contributions. The ratiometric BVPRS2 signal from light scattering is independent of monochromator bandwidth, but fluorescence increases linearly with the monochromator bandwidth.

Xu, J. X.; Hu, J.; Zhang, D.*, Quantification of Material Fluorescence and Light Scattering Cross Sections Using Ratiometric Bandwidth-Varied Polarized Resonance Synchronous Spectroscopy. Anal. Chem., 2018, 90, 7406. (DOI: 10.1021/acs.analchem.8b00847)

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