Both photon absorption and scattering contribute to the photon extinction measured with UV-vis spectrophotometers. However, only absorption extinction induces strong inner-filter-effect (IFE) while extinction by light scattering has no significant IFE. Therefore, one should always decompose the sample UV-vis extinction spectrum into its absorption and scattering component spectra before IFE correction. Find out more research by the Zhang Group.
Xu, J. X.; Vithanage, B. C. N.; Athukorale, S. A.; Zhang, D.*, “Scattering and absorption differ drastically in their inner filter effects on fluorescence, resonance synchronous, and polarized resonance synchronous spectroscopic measurements.” Analyst. 2018, 143, 3382-3389. (DOI: 10.1039/C8AN00790J )
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