Determination of Xylitol in Sugar-Free Gum by GC–MS with Direct Aqueous Injection: A Laboratory Experiment for Chemistry Students

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• Xylitol is generally considered safe for human consumption and is frequently used in sugar free gum; however, it is extremely toxic to dogs.
•  In this laboratory experiment, upper-level undergraduate chemistry students extract xylitol from both fresh and chewed gum sticks followed by direct aqueous injection GC-MS analysis.
• Students learn the proper steps and techniques required for sample extraction and preparation, GC-MS analysis, and determine concentrations of xylitol present in gum samples. Identification
• Upon quantification of xylitol in chewed and unchewed gum samples, students are able to calculate the level of hazard for dogs upon ingestion.

Rajapaksha SM, Samarasekara D, Brown JC, Howard L, Gerken K, Archer T, Lathan P, Mlsna T, Mlsna D. Determination of Xylitol in Sugar-Free Gum by GC–MS with Direct Aqueous Injection: A Laboratory Experiment for Chemistry Students. Journal of Chemical Education. 2018 Sep 27.


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