MS Defense - Weiyu Peng

June 22, 2021

10:00 am


Kinetic Spectroscopic Quantification of Biomarkers in Practical Samples

Weiyu Peng

Department of Chemistry
Mississippi State University

Tuesday, June 22, 2021
10:00 AM
Library Auditorium 1000

Abstract

     The kinetic spectroscopic quantification refers to a subset of chromogenic (CG) and fluorogenic (FG) assays that deduce the analyte concentration basing on the UV-vis or fluorescence signal obtained during the CG/FG reaction processes. Existing kinetic spectroscopic quantification are based predominantly on reactions that can be approximated as a first-order process. Presented in this thesis is the kinetic spectroscopic quantification that uses higher order CG/FG reactions where the overall reaction can be approximated as combination of two sequential first-order processes. Included in chapter one is the theoretical model and several proof-of-concept applications. This model analyte is malondialdehyde (MDA), a broadly interested lipid peroxidation biomarker. Chapter two describes the study of the effects of the reaction solvent, temperature, acid catalyst, and calibration method on the assay performance. The most rapid MDA assays achieved so far is 3 mins, 30 times more efficient than the current equilibrium spectroscopic quantification.


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