Biomedical Raman Spectroscopy Current Laboratory Results and Progress Towards Clinical Applications

Friday, September 29, 2017


Dr. Richard Dluhy

Department of Chemistry

University of Alabama Birmingham

Richard DluhyHand Lab 1144, 3:30 PM.

Development of diagnostic methods for rapid and sensitive biomedical applications is essential for the advancement of therapeutic and intervention strategies necessary to protect public health. Current diagnostic methods, e.g. culture, isolation, PCR, antigen detection, and serology, are often time-consuming, cumbersome, or lack sensitivity.  The current presentation will highlight two aspects of our current work in this area. 

First, we have investigated several different nanoparticle platforms for surface-enhanced Raman (SERS)-based identification and classification of pathogens. These platforms included metal colloids, nanosphere arrays, OAD nanorod arrays, and layer-by-layer nanoparticle assembly. The current talk will address the development of spectroscopic methods for pathogen detection based on these nanostructured SERS platforms.   This presentation will describe the use of these nanofabricated arrays in conjunction with SERS for direct detection of the respiratory pathogen Mycoplasma pneumoniae.  An overview of the challenges and successes that have marked progress toward a real-time SERS-based diagnostic platform for these bacteria is described, as well as strategies employed to address future clinical applications.

Secondly, we have recently employed resonance Raman spectroscopy (RRS) to the problem of in-situ evaluation of the therapeutic efficacy and safety of stored red blood cells (RCs). The quality and safety of stored red blood cells before transfusion remains a key question in transfusion medicine. Resonance Raman spectroscopy (RRS) is a label free modality that provides enhanced vibrational modes associated with molecular electronic transitions in resonance with the excitation wavelength. RRS is not widely used for in-situ applications, since the use of excitation wavelengths in the UV-Vis region increases the risk of photodegradation of the samples and limits the depth of penetration. We recently developed a novel technique, Diffuse Resonance Raman spectroscopy (DRRS), for rapid identification of spectral markers associated with the oxygenation state of hemoglobin. We have combined DRRS with multivariate statistical analysis to classify the age and oxygenation state of RBCs with >95% sensitivity and specificity. Based on these results, DRRS has the potential to be a rapid, non-invasive, and non-destructive approach for in-situ applications targeting molecules such as heme proteins and carotenoids.

Dr. Richard A. Dluhy is currently Professor of Chemistry and Chair of the Department of Chemistry at the University of Alabama at Birmingham.  He received his B.S. degree in Biochemistry from the University of Connecticut, and his Ph.D. in Physical Chemistry from Rutgers University.  He was an NRC Post-Doctoral Research Associate at the National Research Council of Canada in Ottawa, Canada.  Prior to his academic career, Dr. Dluhy worked in the pharmaceutical industry at Hoffman-LaRoche (Nutley, NJ), and in the Biotechnology and Chemical Methods Development groups at Battelle Memorial Institute (Columbus, Ohio).  Prior to arriving at UAB, he was Associate Professor and Professor in the Department of Chemistry at the University of Georgia (Athens), where he was also Associate Department Head and Director of the Nanoscale Science and Research Center. His research interests span a range of topics related to biomedical spectroscopy, and surface and interfacial analysis, particularly the development and application of infrared and Raman spectroscopy to the study the biomolecular assemblies, and chemically assembled nanomaterials at surfaces. Dr. Dluhy’s research has been supported by the National Institutes of Health, National Science Foundation, Howard Hughes Medical Institute, US Department of Energy and the European Union.  His work has been internationally recognized through numerous invited lectures; in addition, he was appointed Professeur Visité, Centre de Physique Moléculaire Optique et Hertzienne at the Université Bordeaux, Bordeaux, France, was awarded a Alexander von Humboldt Research Award from the Humboldt Foundation in Bonn, Germany, and was a DAAD Fellow at the Robert Koch Institute, Berlin, Germany.

 


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